Vitamin D

A simple blood test that checks your vitamin D stores to support bones, muscles, and overall wellbeing.

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Vitamin D
 test

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Test Summary

Sample Type 
Blood
Required Blood
1 mL
Analysis Type
Serum
Collection method
In person at the lab; at-home where available

Why test

Vitamin D

?

Clinicians order vitamin D to check overall status, especially for bone health, muscle function, or fatigue. It helps guide supplement needs, monitor treatment, and assess risk in people with little sun exposure, darker skin, aging, pregnancy, or gut and kidney conditions. It is also checked when calcium or parathyroid hormone levels are off. You can test this marker with Aniva across Germany and Finland.

What is

Vitamin D

?

Clinicians order vitamin D to check overall status, especially for bone health, muscle function, or fatigue. It helps guide supplement needs, monitor treatment, and assess risk in people with little sun exposure, darker skin, aging, pregnancy, or gut and kidney conditions. It is also checked when calcium or parathyroid hormone levels are off. You can test this marker with Aniva across Germany and Finland.

What insights will i get from

Vitamin D

?

High: Often due to too much supplementation and may be linked with high calcium. Review your dose, pause megadoses, and discuss a plan with your clinician.
Low: Common, and may reflect limited sun, low intake, higher needs, or absorption issues. Consider diet, safe sun exposure, or supplements, and recheck after some weeks. Cutoffs vary by lab and guideline—focus on trends and your overall context.

Sample type & collection

  • Sample: Blood
  • Fasting: No
  • Best timing: Morning; repeat in the same season for trends
  • Collection: Standard venous draw into serum separator tube
  • Typical volume: 1 mL
  • Analysis: Serum; LC-MS/MS or immunoassay
  • Prep tips: List supplements; avoid high-dose biotin for 48 hours
  • Turnaround: 1-3 working days

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Vitamin D

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Vitamin D
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Limits & interferences with

Vitamin D

:

Common factors that can affect results include recent high-dose vitamin D supplements, seasonal sunlight changes, sunscreen use, skin tone, higher body weight, aging, pregnancy, and certain medicines (anticonvulsants, glucocorticoids, rifampin, orlistat, cholestyramine, antiretrovirals). Large doses of biotin can interfere with some immunoassays; avoid for 48 hours before testing. Acute illness or inflammation may also shift levels.

Special situations include chronic kidney disease, parathyroid disorders, pregnancy, and malabsorption—confirm or adjust with repeat testing, calcium/PTH, or a 1,25-dihydroxyvitamin D test when advised.

Questions about

Vitamin D

:

What does my vitamin D result mean? It reflects your body’s vitamin D stores. Low levels suggest you may need more intake, sun, or a supplement plan.

Do I need to fast for this test? No. You can eat and drink normally unless your clinician gives other instructions.

What can affect my result? Recent supplements, sun exposure, season, skin tone, age, pregnancy, and some medicines can change levels. High-dose biotin may skew some tests.

How often should I test? If starting or changing supplements, or at higher risk, consider retesting after a few months or as advised by your clinician.

How long do results take? Most labs report results within 1-3 working days.

What should I discuss with my clinician? Your symptoms, diet, sun habits, and medications, plus whether to adjust supplements and recheck calcium or PTH.

Sources:

  • Endocrine Society — Vitamin D for the Prevention of Disease: Clinical Practice Guideline. (2024). https://www.endocrine.org/clinical-practice-guidelines/vitamin-d-for-prevention-of-disease
  • NIH Office of Dietary Supplements — Vitamin D Fact Sheet for Health Professionals. (2024). https://ods.od.nih.gov/factsheets/VitaminD-HealthProfessional/
  • MedlinePlus — Vitamin D Test. (2024). https://medlineplus.gov/lab-tests/vitamin-d-test/
  • ARUP Consult — Vitamin D Testing. (2023). https://arupconsult.com/content/vitamin-d-testing
  • NICE — Vitamin D: supplement use in specific population groups. (2022). https://www.nice.org.uk/guidance/ph56
Medical disclaimer: This content is for informational purposes only and is not medical advice. Always discuss results with a qualified healthcare professional.

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